Department of Dermatology,
Venerology and Allergology
Charité – Universitätsmedizin

Determination of physiological skin parameters and of skin lesions

Research topics:

Determination of physiological skin parameters such as transepidermal water loss (TEWL), pH, Stratum corneum hydration, sebumetry, concentration of antioxidants, analysis of skin roughness and melanin distribution, in vivo characterization of the skin at a cellular level, characterization of wound healing and inflammatory processes, determination of collagen and elastin structures in the skin, analysis of the phase distribution (o/w or w/o) of formulations in the product and on the skin, in vivo analysis of the changes in the lipid structure of the skin during dermatological and cosmetic treatment, analysis of the efficacy of barrier creams and of barrier recovery, ethnic differences of skin parameters.


Skin barrier characterisation using in vivo laser scanning microscopy – healthy skin barrier
  • TEWL
  • pH-measurement
  • Corneometry
  • Sebumetry
  • Mexameter
  • Chromameter
  • In vivo laser scanning microscopy
  • Analysis of the skin surface structure (skin roughness)
  • Optical coherent tomography
  • Two-photon microscopy combined with FLIM and CARS (in vivo, ex vivo, in vitro)
  • Confocal Raman microscopy combined with SERS (in vivo, ex vivo, in vitro)
  • Resonance Raman spectroscopy
Agar Platte
Skin barrier characterisation using in vivo laser scanning microscopy – disturbed skin barrier




M. Klemp, M.E. Darvin, J. Lademann, M. Weinigel, M.C. Meinke.
Comparison of morphologic criterias for actinic keratosis and squamous cell carcinoma with in vivo multiphoton tomography.
Experimental Dermatology. DOI: 10.1111/exd.12912.

J. Schleusener, P. Gluszczynska, C. Reble, I. Gersonde, J. Helfmann, J. W. Fluhr, J. Lademann, J. Röwert-Huber, M. C. Meinke,
In vivo study for the discrimination of cancerous and normal skin using fiber probe based Raman spectroscopy,
Exp Dermatol, 2015, 24, 767-772

C. Czekalla, K.H. Schönborn, S. Markworth, M. Ulrich, D. Göppner, H. Gollnick, J. Röwert-Huber, M.E. Darvin, J. Lademann, M.C. Meinke,
Technical parameters of vertical in vivo multiphoton microscopy: a critical evaluation of the flyscanning method,
Laser Phys. Lett. 12 (2015) 085602 (7pp)

R. Fölster-Holst, J.Galecka , S.Weissmantel, U.Dickschat, F. Rippke, K.Bohnsack, T.Werfel, K.Wichmann, M.Buchner, T. Schwarz, A.Vogt, J.Lademann, M. C. Meinke,
Birch pollen influence the severity of atopic eczema – Prospective clinical cohort pilot study and ex-vivo penetration study,
Clin Cosmetic Invest Dermatol 2015:8 539-548

M.E. Darvin, H. Richter, Y.J. Zhu, M.C. Meinke, F. Knorr, S.A. Gonchukov, K. Koenig, J. Lademann.
Comparison of in vivo and ex vivo laser scanning microscopy and multiphoton tomography application for human and porcine skin imaging.
Quantum Electronics, 44(7): 646-651, 2014.

M.E. Darvin, W. Sterry, J. Lademann (2013).
Optical methods for non-invasive determination of carotenoids in human skin.
J Biomed Optics, 18 (6): 61230.

M. Ulrich, M. Klemp, M.E. Darvin, K. König, J. Lademann, M.C. Meinke (2013).
In vivo detection of basal cell carcinoma: Comparison of a reflectance confocal microscope and a multiphoton tomograph.
J Biomed Opt, 18 (6): 61229.

H.G. Breunig, M. Weinigel, R. Bückle, M. Kellner-Höfer, J. Lademann, M.E. Darvin, W. Sterry, K. König (2013).
Clinical CARS and multiphoton imaging of human skin with spectrally broadened fs pulses.
Laser Phys Lett, 10: 025604.

M.E. Darvin, C. Sandhagen, W. Koecher, W. Sterry, J. Lademann, M.C. Meinke (2012).
Comparison of two methods for noninvasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy.
J Biophotonics, 5 (7): 550-558.

H.B. Vierck, M.E. Darvin, J. Lademann, A. Reiβhauer, A. Baack, W. Sterry, A. Patzelt (2012).
The influence of endurance exercise on the antioxidative level of the skin.
Eur J Appl Physiol, 112 (9): 3361-3367.

N. Luther, M.E. Darvin, W. Sterry, J. Lademann, A. Patzelt (2012).
Ethnic differences in skin physiology, hair follicle morphology and follicular penetration.
Skin Pharmacol Physiol, 25 (4): 182-191.

A. Patzelt, W. Sterry, J. Lademann (2010).
In vivo measurements of skin barrier: Comparison of different methods and advantages of laser scanning microscopy.
Laser Phys Lett, 7 (12): 843-852.

C. Antoniou, J. Lademann, H. Richter, S. Astner, A. Patzelt, L. Zastrow, W. Sterry, S. Koch (2009).
Analysis of the melanin distribution in different ethnic groups by in vivo laser scanning microscopy.
Laser Phys Lett, 6 (5): 393-398.

J. Lademann, A. Patzelt, H. Richter, S. Schanzer, W. Sterry, A. Filbry, K. Bohnsack, F. Rippke, M. Meinke (2009).
Comparison of two in vitro models for the analysis of follicular penetration and its prevention by barrier emulsions.
Eur J Pharm Biopharm, 72: 600-604.

S. Astner, K. Swindells, S. González, E. Stockfleth, J. Lademann (2008).
Confocal microscopy: Innovative diagnostic tools for monitoring of noninvasive therapy in cutaneous malignancies.
Drug Discov Today Dis Mech, 5 (1): 81-91.

J. Lademann, J. Shevtsova, A. Patzelt, H. Richter, N. D. Gladkowa, V. M. Gelikonov, S. A. Gonchukov, W. Sterry, A. M. Sergeev, U. Blume-Peytavi (2008).
Optical coherent tomography for in vivo determination of changes in hair cross section and diameter during treatment with glucocorticosteroids-a simple method to screen for doping substances?
Skin Pharm Phys, 21: 312-317.

T. Rieger, A. Teichmann, H. Richter, S. Schanzer, W. Sterry, J. Lademann (2007).
Evaluation of barrier creams-introduction and comparison of 3 in vivo methods.
Contact Dermatitis, 56: 347-354.

S. Mangelsdorf, N. Otberg, H. I. Maibach, R. Sinkgraven, W. Sterry, J. Lademann (2006).
Ethnic variation in vellus hair follicle size and distribution.
Skin Pharmacol Physiol, 19: 159-167.

M.E. Darvin, A. Patzelt, F. Knorr, U. Blume-Peytavi, W. Sterry, J. Lademann (2008).
One-year study on the variation of carotenoid antioxidant substances in the living human skin: Influence of dietary supplementation and stress factors.
J Biomed Optics, 13 (4): 044028-(1-9).