Department of Dermatology,
Venerology and Allergology
Charité – Universitätsmedizin

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Interaction of antioxidants and free radicals in the skin
(in-vivo, in-vitro, ex-vivo)

Research topics:

Detection of antioxidants in the skin after topical or systemic application, determination of the kinetics of antioxidants in human skin after application of exogenous stress factors, characterization of topically and systemically applied substances (e. g., extracts of herbs, fruit and vegetables) concerning their protection efficiency against the action of free radicals in the skin, determination of the interaction of natural antioxidants of human skin with topically or systemically applied antioxidant substances, protection of sunscreens against radical formation in human skin subsequent to irradiation in different spectral ranges


Raman spectrometer
EPR L-band spectrometer



S. Vandersee, M. Beyer, J. Lademann, M.E. Darvin. Blue-Violet light irradiation dose-dependently decreases carotenoids in human skin, which indicates the generation of free radicals. Oxidative Medicine and Cellular Longevity, 579675, 2015.

M.C. Meinke, R. Müller, A. Bechtel, S.F. Haag, M.E. Darvin, S.B. Lohan, F. Ismaeel, J. Lademann. Evaluation of carotenoids and reactive oxygen species in human skin after UV irradiation: a critical comparison between in vivo and ex vivo investigations. Experimental Dermatology, 24(3): 194-197, 2015.

H. Lademann, B. Gerber, D.M. Olbertz, M.E. Darvin, L. Stauf, K. Ueberholz, V. Heinrich, J. Lademann, V. Briese. Non-invasive spectroscopic determination of the antioxidative status of Gravidae and Neonates. Skin Pharmacology Physiology, 28: 189-195, 2015.

R.-X. Yu, W. Köcher, M.E. Darvin, M. Büttner, S. Jung, B.-N. Lee, C. Klotter, K. Hurrelmann, M.C. Meinke, J. Lademann (2014). Spectroscopic biofeedback on cutaneous carotenoids as part of a prevention program could be effective to raise health awareness in adolescents. J Biophotonics, DOI: 10.1002/jbio.201300134.

M.J. Ahalaya, G.V. Maksimov, A.B. Rubin, J. Lademann, M.E. Darvin (2014). Molecular action mechanisms of solar infrared radiation and heat on human skin. Ageing Res Rev, DOI: 10.1016/j.arr.2014.03.006.

M.E. Darvin, W. Sterry, J. Lademann. Optical methods for non-invasive determination of carotenoids in human skin (2013). J Biomed Opt, 18 (6): 061230-(1-9).

M.E. Darvin, W. Sterry, J. Lademann, A. Patzelt (2013). Alcohol consumption decreases the protection efficiency of the antioxidant network and increases the risk of sunburns in human skin. Skin Pharmacol Physiol, 26: 45-51, 2013.

M.C. Meinke, A. Friedrich, K. Tscherch, S.F. Haag, M.E. Darvin, H. Vollert, N. Groth, J. Lademann, S. Rohn (2013). Influence of dietary carotenoids on radical scavenging capacity of the skin and skin lipids. Eur J Pharm Biopharm, 84: 365–373.

A. Lauer, N. Groth, S.F. Haag, M.E. Darvin, J. Lademann, M.C. Meinke (2013). Radical scavenging capacity in human skin before and after vitamin C uptake: An in vivo Feasability study using electron paramagnetic resonance spectroscopy, J Invest Dermatol, 133 (4): 1102-1204.

S. Arndt, S.F. Haag, A. Kleemann, J. Lademann, M.C. Meinke (2013). Radical protection in the visible and infrared by hyperforin rich cream - in vivo versus ex vivo methods. Exp Dermatol, 22 (5): 354-357.

M.E. Darvin, W. Sterry, J. Lademann, T. Vergou (2011). The role of carotenoids in human skin. Molecules, 16 (12): 10491-10506.

J. Lademann, S. Schanzer, M. Meinke, W. Sterry, M.E. Darvin (2011). Interaction between carotenoids and free radicals in human skin. Skin Pharmacol Physiol, 24: 238–244.

M.E. Darvin, J.W. Fluhr, M.C. Meinke, L. Zastrow, W. Sterry, J. Lademann (2011). Topical beta-carotene protects against infra-red-light induced free radicals. Exp Dermatol, 20 (2): 125-129.

M.C. Meinke, S.F. Haag, S. Schanzer, N. Groth, I. Gersonde, J. Lademann (2011). Radical protection by sunscreens in the infrared spectral range. Photochem Photobiol, 87(2): 452-456.

M.E. Darvin, W. Sterry, J. Lademann (2010). Resonance Raman spectroscopy as an effective tool for the determination of antioxidative stability of cosmetic formulations. J Biophotonics, 3 (1-2): 82-88.

M.C. Meinke, M.E. Darvin, H. Vollert, J. Lademann (2010). Bioavailability of natural carotenoids in human skin compared to blood. Eur J Pharm Biopharm, 76 (2): 269-274.

M.E. Darvin, S.F. Haag, L. Zastrow, W. Sterry, J. Lademann, M.C. Meinke (2010). Formation of free radicals in human skin during irradiation with infrared light, J Invest Dermatol, 130 (2): 629-631.

M.E. Darvin, S. Haag, M. Meinke, L. Zastrow, W. Sterry, J. Lademann (2010). Radical production by infrared-A irradiation in human tissue. Skin Pharmacol Physiol, 23: 40-46.

P. Schroeder, J. Lademann, M.E. Darvin, H. Stege, C. Marks, S. Bruhnke, J. Krutmann (2008). Infrared radiation-induced matrix metalloproteinase in human skin: Implications for protection. J Invest Dermatol, 128 (10): 2491-2497.

M.E. Darvin, I. Gersonde, H. Albrecht, W. Sterry, J. Lademann (2006). In-vivo Raman spectroscopic analysis of the influence of UV radiation on carotenoid antioxidant substance degradation of the human skin. Laser Phys, 16 (5): 833-837.