Department of Dermatology,
Venerology and Allergology
Charité – Universitätsmedizin

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Development and evaluation of anti-aging strategies

Research topics:

Analysis of the efficiency of topically applied anti-aging creams and sunscreens, determination of their protection properties (absorption, reflection, etc.) and the efficacy of neutralization of free radicals produced by solar radiation and by other stress factors in the skin, analysis of changes in the collagen and elastin structure during topical or systemic treatment or influence of stress factors, analysis of the systemic, topical and combined treatment of the skin with antioxidants contained in nutrition supplements, evaluation of the intensity of the influence of stress factors on the skin.


Anti Aging
In vivo analysis of the collagen and elastin in human skin
  • In vivo resonance Raman spectroscopy
  • In vivo Raman microscopy
  • Two photon microscopy
  • electron paramagnetic resonance spectroscopy
  • UV-VIS- NIR spectroscopy
  • Analysis of the skin surface structure (skin roughness)
  • Confocal Raman microscopy
Anti Aging
Skin surface structure of aged skin


J. Lademann, T. Vergou, M.E. Darvin, A. Patzelt, M.C. Meinke, C. Voit, D. Papakostas, L. Zastrow, W. Sterry, O. Doucet,
Influence of topical, systemic and combined application of antioxidants on the barrier properties of the human skin,
Skin Pharm Physiol 2016;29:41-46

M.E. Darvin, H. Richter, S. Ahlberg, S.F. Haag, M.C. Meinke, D. Le Quintrec, O. Doucet, J. Lademann (2014).
Influence of sun exposure on the cutaneous collagen/elastin fibers and carotenoids: Negative effects can be reduced by application of sunscreen.
2014 Sep;7(9):735-43.

S.F. Haag, K. Tscherch, S. Arndt, A. Kleemann, I. Gersonde, J. Lademann, S. Rohn, M.C. Meinke (2014).
Enhancement of skin radical scavenging activity and stratum corneum lipids after the application of a hyperforin-rich cream.
Eur J Pharm Biopharm, 86 (2): 227-233.

M.E. Darvin, W. Sterry, J. Lademann, A. Patzelt (2013).
Alcohol consumption decreases the protection efficiency of the antioxidant network and increases the risk of sunburns in human skin.
Skin Pharmacol Physiol, 26: 45-51.

J. Lademann, M. Meinke, W. Sterry, M.E. Darvin (2011).
Carotenoids in human skin.
Exp Dermatol, 20 (5): 377-382.

J. Lademann, S. Schanzer, M. Meinke, W. Sterry, M.E. Darvin (2011).
Interaction between carotenoids and free radicals in human skin.
Skin Pharmacol Physiol, 24 (5): 238-44.

M.E. Darvin, J.W. Fluhr, M.C. Meinke, L. Zastrow, W. Sterry, J. Lademann (2011).
Topical beta-carotene protects against infra-red-light induced free radicals.
Exp Dermatol, 20 (2): 125-129.

M.E. Darvin, S.F Haag, M. Meinke, L. Zastrow, W. Sterry, J. Lademann (2011).
Determination of the influence of IR radiation on the antioxidative network of the human skin.
J Biophotonics, 4 (1-2): 21-29.

J. Lademann, P.J. Caspers, A. van der Pol, H. Richter, A. Patzelt, L. Zastrow, M. Darvin, W. Sterry, J.W. Fluhr (2009).
In vivo Raman spectroscopy detects increased epidermal antioxidative potential with topically applied carotenoids.
Laser Phys Lett, 6 (1): 76-79.

U. Blume-Peytavi, A. Rolland, M.E. Darvin, I. Pineau, C. Voit, K. Zappel, G. Schäfer, M. Meinke, W. Sterry, J. Lademann (2009).
Cutaneous lycopene and β-carotene levels measured by resonance Raman spectroscopy: High reliability and sensitivity to oral lactolycopene deprivation and supplementation.
Eur J Pharm Biopharm, 73 (1): 187-194.

P. Schroeder, J. Lademann, M.E. Darvin, H. Stege, C. Marks, S. Bruhnke, J. Krutmann (2008).
Infrared radiation-induced matrix metalloproteinase in human skin: Implications for protection.
J Invest Dermatol, 128 (10): 2491-2497.

M. Darvin, A. Patzelt, S. Gehse, S. Schanzer, C. Benderoth, W. Sterry, J. Lademann (2008).
Cutaneous concentration of lycopene correlates significantly with the roughness of the skin.
Eur J Pharm Biopharm, 69: 943-947.